Mesocosm 2019 Hatchlings 20190813

Looking at the hatching data for weeks 4 through 7. First looking at the number of hatchlings that emerged prior to and post vernalization broken down by the week the ephippia where sampled, and faceted out by clone.

 Looking at the same data but as proportions.

Overall it looks like the proportion of hatchlings emerging after vernalization is decreasing over time, because more individuals are emerging prior to vernalization.

Next I wanted to look at how survival to reproduction varies between vernalized and non-vernalized hatchlings. Here we are only looking at data from weeks 4-6, because week 7 hatchlings have only been around for 1 week. This is looking at the number of hatchlings that died prior to reproduction (1, green) versus those that survived to reproduction (0, red). Sorry if this is counterintuitive. Broken down by sample week and clone.

Now let's look at the interaction of vernalization and mortality prior to reproduction. The x-axis is vernalization status, with 1 (right) corresponding to individuals that emerged post vernalization, and 0 (left) corresponding to individuals that emerged prior to vernalization. The bars are broken down by survival, with red (0) being individuals that did not die prior to reproduction (survived to reproduce), and blue (1) being individuals that did die prior to reproduction. Again broken up by clone. Ignore that 6th panel. Sorry about that.

Same data but now looking at it as proportion of individuals that died prior to reproduction.

As we had talked about before, it looks like there is a tendency for higher mortality in individuals that emerged prior to vernalization. Interestingly (maybe) is that the numbers look pretty similar for AxB, D8222, and D8515.

I was also curious about how sterility might change with vernalization. Unfortunately for this data we can only look at sample weeks 4 and 5, because they are the only set of vernalized individuals to make it to week 5, where sterility is scored. The x-axis is again vernalization status. Now the bars are colored as red (0) if fertile and green (1) if sterile. Unfortunately the sample size for the non-vernalized individuals is pretty low here. So not sure we can say much. May need to wait for the data from the next set of vernalized individuals.

 Same data but looking at proportion of sterile individuals.

Next I wanted to look a bit at male production. Here I am only looking at the non-vernalized individuals from weeks 4-7, because they have been in the experiment long enough I feel like they have had a good chance to make males. the x-axis is sample week, and the green (1) corresponds to clones that were observed to make at least 1 male, while red (0) corresponds to clones that have yet to have a single male observed (could still be present, as the entire jar is not checked). 

 Same data but looking at proportion of individuals that have been observed to produce males.