Vernalized versus Non-vernalized Hatchlings Mesocosms 2019

We collected ephippia at sample weeks 4, 5, 6, 7, and 8. So far the plates from weeks 4, 5, and 6 have come out of the fridge. So we have both vernalized and non-vernalized hatchling counts for these three weeks. The plates from sample weeks 7 and 8 will come out of the fridge a week from today (August 2nd). So we only have non-vernalized hatchling counts for those two weeks.

I took an initial look at vernalized versus non-vernalized hatchling counts for weeks 4, 5, and 6. First, I looked at the overall counts of vernalized versus non-vernalized hatchlings by sample week (summing across all tanks/clones). Here is what I get.

Hmm, resolution is awful. But the green bars are vernalized hatchlings, and the red bars are non-vernalized hatchlings. The y-axis scale goes from 0 to 400, with ticks at 100. The x axis is weeks 4, 5, and 6. From this it looks like the ratio of vernalized to non-vernalized hatchlings decreases with sample week (i.e. fewer individuals appear to require vernalization).

We can also look at this by proportion of hatchlings that were emerged after vernalization by sample week.

Again, the resolution is bad (need to figure out the new screenshot program on my new operating system, looks crappy right now). But, the x-axis is again sample week, with weeks 4, 5, and 6. The y-axis is now proportion of hatchlings that were emerged post-vernalization. Just like in the previous graph we see a decrease in this proportion with increasing sample week.

Then I broke it up by clone (D8515, D8222, D8179, D8349, and AxB). Here are what the results look like.

We see a similar pattern as when looking across all clones/tanks. But the patterns seems the most marked in the AxB tanks. 

We can break this down even more by tank and replicate.

Still seem the same pattern mostly holding true.