Phenotyping and Survival by SuperClone
We had been wanting to look at whether ability to phenotype a clone, or survival of a clone, covaries with super clone assignment. Here are the results from some initial work on the data in excel (I know you hate excel, but it was easiest for me for an initial look.)
Here is data for looking at whether clones were phenotypes or not based on super clone. I am only including data from 2017 D8 and DBunk. The two bars on the far right are looking at the other/unique clones from the two ponds. I grouped them all together here.
Now looking at whether the clones are still alive, or are dead/missing.
Now looking at all 2017 data for survival.
From this there certainly seems to be variation among the super clones in terms of whether we have been able to phenotype them and whether they are alive. Super clone A, overall seems to be doing better than super clone B (those are the two main clones in D8). Super clone D from D Bunk did badly across the board, while H and I did better.
I also looked at the percentage of clones that could be phenotyped and/or survived based on whether they belonged to super clones or were unique clones.
Little more uncertain here (and no error bars or stats, so not sure how much you can say). But just from the pattern clones that belong to super clones are more likely to have been phenotyped and are more likely to have survived. Of course most of D8 clones are super clones. If you do the same thing but only look at clones from DBunk we see a different story (though the numbers are also much smaller, as we have phenotypes substantially more D8 than DBunk clones). In DBunk, at least so far, we are about as likely to phenotype unique clones as super clone clones. So far though this is only a small sample size as I only genotyped 38 DBunk clones so far, 11 of those have been phenotyped and 27 have not.
In sum, not sure how much we can say at this point, but it does seem there is variation in performance (happiness, survival) among super clones. Would you agree?
I know this may seem like procrastination from writing my NRSA, but was hoping this might give me some ideas on questions to ask.
Here is data for looking at whether clones were phenotypes or not based on super clone. I am only including data from 2017 D8 and DBunk. The two bars on the far right are looking at the other/unique clones from the two ponds. I grouped them all together here.
Now looking at whether the clones are still alive, or are dead/missing.
Now looking at all 2017 data for survival.
From this there certainly seems to be variation among the super clones in terms of whether we have been able to phenotype them and whether they are alive. Super clone A, overall seems to be doing better than super clone B (those are the two main clones in D8). Super clone D from D Bunk did badly across the board, while H and I did better.
I also looked at the percentage of clones that could be phenotyped and/or survived based on whether they belonged to super clones or were unique clones.
Little more uncertain here (and no error bars or stats, so not sure how much you can say). But just from the pattern clones that belong to super clones are more likely to have been phenotyped and are more likely to have survived. Of course most of D8 clones are super clones. If you do the same thing but only look at clones from DBunk we see a different story (though the numbers are also much smaller, as we have phenotypes substantially more D8 than DBunk clones). In DBunk, at least so far, we are about as likely to phenotype unique clones as super clone clones. So far though this is only a small sample size as I only genotyped 38 DBunk clones so far, 11 of those have been phenotyped and 27 have not.
In sum, not sure how much we can say at this point, but it does seem there is variation in performance (happiness, survival) among super clones. Would you agree?
I know this may seem like procrastination from writing my NRSA, but was hoping this might give me some ideas on questions to ask.





So, it sort of looks like the super clones are healthier in the lab, at least anecdotally.
ReplyDeleteSome thoughts have come to mind about lab meeting yesterday. For instance, are the clones in DBunk hybrids between the two super clones in D8?
That is a really intriguing idea. Is there a quick, relatively easy way to check if they are the result of hybridization between the main clones in D8? It should be some sort of admixture assay?
DeleteIf you ran a STRUCTURE-like analysis focusing on D8 & DBunk you might be able to pick it up fairly quickly.
DeleteThat paper that I send you this AM outlines several programs you could use. I would probably suggest ADMIXTURE. https://www.genetics.ucla.edu/software/admixture/download.html
You would need to convert you VCF file to PLINK format, but that is fairly easy to do with PLINK 1.9